مقاله The Effects of Low Level Laser Therapy on the Expression of Collagen Type I Gene and Proliferation of Human Gingival Fibroblasts (Hgf3-Pi 53): in vitro Study

مقاله The Effects of Low Level Laser Therapy on the Expression of Collagen Type I Gene and Proliferation of Human Gingival Fibroblasts (Hgf3-Pi 53): in vitro Study

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مقاله The Effects of Low Level Laser Therapy on the Expression of Collagen Type I Gene and Proliferation of Human Gingival Fibroblasts (Hgf3-Pi 53): in vitro Study دارای 5 صفحه می باشد و دارای تنظیمات در microsoft word می باشد و آماده پرینت یا چاپ است

فایل ورد مقاله The Effects of Low Level Laser Therapy on the Expression of Collagen Type I Gene and Proliferation of Human Gingival Fibroblasts (Hgf3-Pi 53): in vitro Study کاملا فرمت بندی و تنظیم شده در استاندارد دانشگاه و مراکز دولتی می باشد.

توجه : در صورت  مشاهده  بهم ريختگي احتمالي در متون زير ،دليل ان کپي کردن اين مطالب از داخل فایل ورد مي باشد و در فايل اصلي مقاله The Effects of Low Level Laser Therapy on the Expression of Collagen Type I Gene and Proliferation of Human Gingival Fibroblasts (Hgf3-Pi 53): in vitro Study،به هيچ وجه بهم ريختگي وجود ندارد


بخشی از متن مقاله The Effects of Low Level Laser Therapy on the Expression of Collagen Type I Gene and Proliferation of Human Gingival Fibroblasts (Hgf3-Pi 53): in vitro Study :

سال انتشار : 2013

تعداد صفحات : 5

Objective(s):
Recent investigations show that both proliferation and secretion of macromolecules by cells can be regulated by low level laser therapy (LLLT). The aim of this study was to determine whether LLLT could induce a bio-stimulatory effects on human gingival fibroblasts (HGF3-PI 53). Therefore, the effect of laser irradiation on human gingival cell proliferation and collagen type I gene expression was studied.

Materials and Methods:
HGF3-PI 53 were cultured in 96-well plate and then irradiated with LLLT gallium-aluminum-arsenide (Ga–Al–As), 810 nm, 50 mW diode laser (energy: 4 J/cm2) for three consecutive days. The cell proliferation was measured on days 1, 2 and 3 after irradiation with LLLT using MTT assay. Real time PCR analysis was utilized on day 3 to evaluate the expression of collagen type I gene.
Results
: Evaluation of cellular proliferation, one day after laser treatment showed no difference compared to control group. But on days 2 and 3, significant increase in proliferation was observed in the irradiated cell populations in comparison to the control group. Treatment of HGF3-PI 53 by laser resulted in a significant increase in collagen I gene expression on 3 day.
Conclusion:
The results demonstrated that LLLT stimulated human gingival fibroblast proliferation as well as collagen type I gene expression in vitro.

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مقاله The Effects of Low Level Laser Therapy on the Expression of Collagen Type I Gene and Proliferation of Human Gingival Fibroblasts (Hgf3-Pi 53): in vitro Study